Fluorimetric Determination Of Methyl-Parathion And Study On The Trace Elements In Achyranthes Bidentatas

It is well known that Chinese traditional medicine plays a great role in the prevention and cure of some disease and makes a strong impact on our health. Although much is known about its function, little is available on heavy metals and pesticides residues in Chinese traditional medicine. The purpose of this study to determine the content of heavy metals and the pesticides residues is of paramount importance to help in assessing the GAP(Good Agricultural Practice) of Chinese traditional medicine. The GAP research of four Huaiyao is a key item of the Tenth Five Years Program of the Ministry of Science & Technology. Achyranthes bidentata (AB) , one of the four Huaiyao, is a famous Chinese traditional medicine. In this paper, we study systemically on the content of heavy metals(Hg, As, Cu, Zn and Fe) and organophosphorus pesticide- methyl-parathion in Achyranthes bidentata of different habitats and different processing methods. The objective of the present study is not only to provide some reference to establish the GAP of Achyranthes bidentata, but also to accelerate the modernization and internationalization of Chinese traditional medicine.The content of this paper is as follows:1. A new method by fluorimetry with calcein-Pd2+ was used to determinate organophosphorus pesticide- methyl-parathion. This method is optimized. The linear range is 1.0X107 -1.2 X 10-6mol/L and the detection limit is 5.0 X 10-8mol/L. Recovery is 87.0-108.0%, RSD < 4.0%.2. The levels of five heavy metals (Hg, As, Cu, Zn and Fe) and one representative organophosphorus pesticide - methyl-parathion have been measured by different methods in 11 different samples of Achyranthes bidentata (AB). A new method by fluorimetry with calcein-Pd2+was used to determinate organophosphorus pesticide- methyl-parathion. Heavy metals such as Hg, As were determined by HG-AFS(hydride generation-atomic fluorescence). Other metals(Cu, Fe, Zn) were determined by FAAS(flame atomic absorbtion spectrophotometry). To the samplescollected from the same habitats, a rule could be found from the results that the levels of methyl-parathion in the samples mix-fried with wine decreased more or less, compared to the raw samples. The values of each individual heavy metal for the different samples were within the same order of magnitude, For the values of five heavy metal in the samples, the order of magnitude of Fe, which was the biggest, presented 102 ug/g, followed by Cu and Zn, both with the order of magnitude of 101 ug/g. Lower orders of magnitude were found for As and Hg, presented 10"1 and 10"2 ug/g, respectively.3. Acetamiprid is a new pesticide which has good function to the drug-fast pests. The formulation of the 20% Acetamiprid Soluble Concentrate is introduced. The best composition as well as specification for its quality control is described, especially it can be diluted with water of 50 times in volume. It has a good foreground on the market. This is a chemical project that we coorperate with ZhengZhou sanonda Co., Ltd.