Preparation Of Monolithic Polymer Column And Its Application To Bio-molecule

In contrast to traditional packed column, monolithic column has the advantage of easy preparation, excellent permeability, low backpressure at chromatographic runs and large capacity for samples. This kind of column has been applied in many fields.This paper consists of the following parts: preparation of monolithic polymer column, investigation of polymerization conditions and its application to bio-molecules.First, a continuous rod hydrophobic interaction chromatographic column consisting of poly(glycidyl methacrylate and ethylene dimethacrylate) was prepared by a free radical polymerization and used in the separation of snailase. The influences of polymerization conditions on the hydrophobility of the rod and on the separation of snailase were investigated.Second, a mathematical model predicting the non-linear retention behavior protein was established. Using this model, the non-linear retention behaviors of different proteins under the gradient elution in high performance hydrophobic interaction chromatographic column have been predicted.Third, a continuous ion-exchange chromatographic column with glycidyl methacrylate as the functional monomer and ethylene dimethacrylate as the cross linker was prepared by a free radical polymerization and applied to the separation of IL-18. After the factors influencing the separation were investigated, an optimum condition for separating EL-18 from its related proteins was obtained.