| The present thesis consisted two parts:1. Review: Application of Chemiluminescence in Environmental, Biochemical and Pharmaceutical Analysis, including more than 100 references concerning Chemiluminescence analysis during 2000 ~ 2004.2. Research reports was based on the research works completed, and the main contents were presented as following:(1) A New Green Analytical Procedure for Monitoring Sub-Nanogram Amounts Chlorpyrifos on Fruits Using Flow-Injection Chemiluminescence with Immobilized Reagents(2) On-line Monitoring of Formaldehyde in Water and Air Using Chemiluminescence Detection(3) Sub-picogram Level Determination for Vitamin B12 in Medicine and Biological Samples with Flow Injection Analysis by Chemiluminescence Detection(4) Flow Through Sensor For the Chemiluminescence Determination of Thiamine(5) Sensitive Determination of Sub-nanogram Amounts of Rutin by Its Inhibition on Chemiluminescence with Immobilized Reagents(6) Chemiluminescence Assay for Uric Acid in Human Serum and Urine Using Flow-injection With Immobilized Reagents TechnologyPart I Application of Chemiluminescence in environmental, pharmaceutical and biological analysisBased on the foundamental theory and reactions of the Chemiluminescence (CL), the applications of Chemiluminescence in environmental, pharmaceutical and biological analysis was reviewed. CL methods have been widely used in the environmental analysis because it took advantages involving ultrasensitivity, instrumental simplicity. Due to the catalytic effects of heavy metal ions on the CL reaction, CL methods were developed for determine Cu2+ and Cr3+ in waste water. By combination with the separate technique, the CL methods for environmental analysis were improved in sensitivity and selectivity. The detection of residues of pesticides in environments became more and more important, and two types of CL methods were described based on Ru(bpy)33+ and luminol-H2O2 CL systems, respectively. And also, the CL methods involving enzymatic reaction were presented in general. Several CL approaches were also established for the poisonous gases in air. SO2 and NOX were usually determined as SO32- and NOOO- in CL methods respectively. Additionally, many hazard gases were determined chemiluminesently by their CL reaction with ozone. The CL method is an important tool in pharmaceutical research, especially quality control, clinical analysis and pharmacology. On the basis of the CL reaction between pharmaceutical analytes and oxidants, such as KMnO4, K3Fe(CN)6, Cerium(IV) and Ru(bipy)33+, several CL methods were proposed. On the other hand, a lot of Pharmaceuticals showed enhancive or inhibitoryeffect on the CL reaction, and such methods were summarized based on the references from 2000 to 2004. The chemiluminescence method for bioanalysis developed very fast in recent years, and enyme-catalytic CL approaches and immunoassay coupling chemiluminescent labels were described briefly.Part II Research reportsThree CL systems, luminol-IO4", vitamin B12-luminol-dissolved oxygen and luminol-Fe(CN)63", were involved in the presented work. Based on these CL systems, several CL analytical procedures were developed by combination with FI and reagent-controlled-release technology, and applied for environmental, pharmaceutical and biological analysis.(1) Chlorpyrifos is a broad-spectrum organophosphorus pesticide. Based on its inhibitory effect on CL generated by luminol with periodate, a novel green method using flow injection chemiluminescence with controlled-reagent-release technology has been developed for monitoring sub-nanogram amounts chlorpyrifos. The analytical reagents involved in chemiluminescence reaction, luminol and periodate, were both immobilized on an anion-exchange column. The decrease of CL intensity was linear over the logarithm of concentration of chlorpyrifos ranging from 0.48 to 484.0 ng-ml"1 (r2 = 0.9969), and the limit of detection is 0.18 ng-ml"1 (3cr), offering the sampling efficiency of 120 h"1. The proposed method w...
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