Studies On Chemical Constituents And Bioactivity Of Salvia Sclarea

It is an important way to search new fungicides from plants. Tested with Botrytiscinerea Fusarium graminearum Phytophthora capsici Exserohilum turcicum Glomerellacingulata Bipolaris maydis and Erysiphe graminis et al 13 kinds of fungi, the bioactivity ofsalvia sclarea was studied by a systematic way in vitro and pot test. The results are asfollows: 1. The flowers of salvia sclarea were extracted with systematic solvents. The bioassayresults indicated that the optimal solvent extracting active components was petroleum ether. Italso showed that the petroleum ether extract could markedly inhibit the various hypha growth,its inhibition ratio to hypha of 6 fungi are more than 70% respectively, its EC50 value againstBotrytis cinerea sclerotinia sderotiorum Alternaria longipes was 77.20 26.98 58.07μg·mL-1 respectively. 2. The seeds and leaves of salvia sclarea were extracted with petroleum ether, ethylacetate, acetone respectively, and the three extracts showed low ihhibition to hypha of 5 fungi. 3. The result of pot test indicated that the protective effect and therapeutic effect of thepetroleum ether extract against the Erysiphe graminis were 94.9% and 84.2% respectively. 4. By the chromatogram, 4 constituents were isolated from petroleum ether extract andethyl acetate extract of the flowers ,which are sclareol, β-sitosterol, dotriacontane,fatty acidrespectively. 5. The bioassay of fungicidal activity showed sclareol had strong inhibition againstsclerotinia sderotiorum Alternaria longipes Botrytis cinerea Bipolaris sorokiniana,whichEC50 were 7.25 11.45 63.57 8.69μg·mL-1. At the concentration of 1000μg·mL-1, the resultof pot test indicated that sclareol had strong inhibition to wheat powdery mildew, protectiveeffect was 86.4%, therapeutic effect was 94.7%. 6. Dotriacontane showed obvious inhibiting to sclerotinia sderotiorum.The inhibitionratio of fatty acid to hypha of 3 fungi were above 60% respectively.Both dotriacontane andfatty acid had strong inhibition to Exserohilum turcicum and Bipolaris sorokiniana.