Isolation Of Alkaline Lipase Producing Strain And Purification And Characterization Of Alkaline Lipase

A strain of lipase producing bacterium was isolated form soil. It is identified as Yersinia van Loghem and named as A6.The optimum temperature of alkaline lipase is 40 ℃ and the optimum pH is 9.0. The molecular weight of the purified alkaline lipase is about 31kD. The optimum conditions of the bacterium fermentation for producing lipase and some properties of the enzyme are also investigated. This enzyme is classified as alkaline lipase. About 80% of the original activity is lost by heating at 70 ℃ for 10 minutes and 80% of the original activity is maintained by heating at 40℃ for 60 minutes.The enzyme activity is stimulated by Ca2+ and Mg2+, but is inhibited by Zn2+, Fe2+, Mn2+ and EDTA. K+ and Na+ have no influence on the enzyme activity.