Study On The Extraction,Purification,Structure Analysis And Biologic Activeof Water-Soluble Polysaccharides From The Fruit Of A.Persica.l.var.Seleropersica

The water-soluble polysaccharides of A.Persica.L.var.seleropersica were extracted in hot water, and then isolated by ethanol. The effect of three factors such as the temperature of extraction, the proportion of hot water volume, the time of extraction on the extraction of the water-soluble polysaccharides were investigated. With the orthogonal design of L9(34), the optimum conditions of extraction were obtained as follow: adding 2 times water as much as fruit weight in 90癈 for 3 hours. The crude polysaccharide was filemot, can soluble in hot water, alkali and acid, cannot soluble in ethanol, acetone, aether etc. The protein in crude polysaccharides was removed by Sevag way and the pigment in crude polysaccharides was removed by H2O2. The water-soluble polysaccharides of peach which color was pure white were got. HTP were further purified to HTP1 and HTP2 with DEAE-Sepharose column chromatography. HTP1 and HTP2 were purified with Sephacryl S-300 column chromatography respectively, however were singular peak. The high performance size-exclude chromatography proved that HTP1 and HTP2 were pure material. The average molecular weight of HTP1 was 52107 and the average molecular weight of HTP2 was 59855. Their structures were investigated by chemical methods (composition analysis, periodate oxidation, and Smith degradation) and spectroscopic methods (UV, IR, NMR). Their monosaccharide was identified by HPLC after TFA hydrolyze. HTP1 is composed of D-glucose, D-galactose, D-Arabinose and L-Rhamnose in the ratio of 1.0: 4.2: 14.5: 1.5, HTP2 iscomposed of D-galactose, D-Mannose and D-Galacturonic acid in the ratio 34.3: 5: 1. The result of periodate oxidation and Smith degradation showed that the major linkage of HTP1 is (1→3) D-galactose and the major linkage of HTP1 is (l→ 3) D-Galacturonic acid. Infrared analysis show that HTP1 and HTP2 were all alpha -glycoside linkage.The crude polysaccharides and the HTP had different effect on antibacterial. The crude polysaccharides can inhibit Escherichia coli, Penicillium citrinum and Aspergillus niger, and HTP can only inhibit Escherichia coli, has no effect on Penicillium citrinum and Aspergillus niger. HTP1 and HTP2 have evident effect in cleaning .OH, the IC50 are 2.87mg/ml and 270.92 n g /ml. HTP1 has evident effect in cleaning O2- ,the IC50 is 401.3 μg /ml. HTP2 has no effect on cleaning O2:. HTP1 and HTP2 were injected into the mice which were inoculated Lewis lung cancer with high dose 200mg/kg and low dose 100mg/kg respective. The result showed that when high dose was injected, the percentage of anti-tumors of HTP1 and HTP2 were 54.69% and 47.08%, when low dose was injected, the percentage of anti-tumors of HTP1 and HTP2 were 43.44% and 44.48%. HTP1 and HTP2 exhibited stimulating activity to the translation of lymphocyte and improving to the activity of NK. These indicated that HTP1 and HTP1 could improve the immunity of the mouse.