Studies On The Preparation Progress And Quality Standard Of Cuijingzhiyun Granules

The compatibility of Misoprosol and Mifepristone is a widely used method of medical induced abortion at present, which complete abortion rate is beyond 90 percent, but the side effect of medicine occurs simultaneously. The original form of Cuijingzhiyun granules is decoction. The clinic experiments have indicated that the compatibility of Cuijingzhiyun granules and Mifepristone has certain effect for termination of early pregnancy and alleviate the side effect of medicine. With the new preparation technology, the thesis has studied the preparation processes and quality standard of granules.Cuijingzhiyun granules consist of Herba Leonuri (Yimucao) , Rhizoma Chuanxiong(Chuanxiong), Radix Angelicae Sinensis(Danggui), Fructus Aurantii(Zhike), Rhizoma Curcumae(Ezhu), Flos Carthami(Honghua) and Hirudo(Shuizhi). The main medicine is Herba Leonuri (Yimucao), the determination of stachydrine hydrochloride in Herba Leonuri was determined by ammonium reineckate spectrophotometry method. Using total alkaloid content and extracts as markers, the optimum extraction technology of Herbal Leonuri and Flos Carthami was selected with the orthogonal experiment. The optimum extraction technology is as follows: adding 12 times 80% ethanof, reflux-extraction 3 times, 90min for each time.By studying volatile oil extraction and the technology process of inclusion compound, the optimum preparation condition of inclusion compound of B-cyclodextrin with volatile oil was that: the ratio of volatile oil to B-CD wasl:8(ml:g),adding 10 times water, stirring 2h at 40C. The inclusion compound formation was proved by microscopical image-forming, DSC and TLC. It was indicated that the character of volatile oil before and after being included was uniform. The methods were accurate and quick, the stability of volatile oil can be obviously raised and the punget smell of volatile oil was covered by the inclusion compound, the state of volatile oil changed to solid from liquid, which was convenient to preparation.Ultramicro pulverization is a new technology that develops very rapidly in recent years.Ultramicro powder of Hirudo was prepared with new methods of ultramicro pulverization. In the experiment of isolated uterus of mice pharmacodynamics of powder and ultramicro powder of Hirudo were compared. As a result, it indicates that ultramicro powder of Hirudo excels powder in the stimulation effect of isolated uterus of mice. At the same time it makes clear that new method of ultramicro pulverization has advantage and wide application foreground.The quality standard of Cuijingzhiyun granules was studied. Applying TLC to indentify Herba Leonuri, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Fructus Aurantii, Using HPLC to determine the content of ferulic acid of Rhizoma Chuanxiong and Radix Angelicae Sinensis. The methods are accurate and quick, and can be used for the quality control of Cuijingzhiyun granules.