Study Of Bacteria-degradation Of Bpa And Degrading Mechanism

Nowadays, endocrine disrupter chemicals are endangering human health. Of all EDCs, Bisphenol A (Bpa) is very important one. With Bpa used in great quantity,it is detected in all kinds of waters all over the world. Control of Bpa pollution is very necessary. So we did some correlative work.First of all, the isolation of strain able to degrade Bpa effectively and classify. A strain (W2) that was isolated ,which can degrade BPA very effectively and grow on saturation solution of Bpa as a sole carbon and energy sources. It was identified as pseudonomas sp.Secondly, study of the cultivation condition of pseudonomas sp. W2. In lab, we researched the effect of cabon source, nitrogen source, pH at beginning, temperature and aerate to the growth of strain W2 and degradation of Bpa. The optimal growth condition: pH7, 30℃, 50ml culture medium in 250ml flask. The optimal Bpa degradation condition: pH7,35℃, 100ml culture medium in 250ml flask.Thirdly, study of Bpa-degrading mechanism of pseudonomas sp.W2. Through the moleculor biology technique, we acknowledged that the Bpa-degrading enzyme is out-cell and the key gene is located at a plasmit about 19kb. Then, we studied the pathway of bacterial metabolism of Bpa and identified three kinds of metabolon of p-Hydroxy benzoic acid , p-Hydroxy benzaldehyde and p-Hydroxy acetophenone.Finally,we amplified and sequenced the pcaG gene.Our study result laid a foundation for exploitation of environmental Bpa-remeding microorganism and for building a gene-engineering microorganism to promote bioremediation of Bpa contamination.