Application Of Capillary Electrophoresis Micro-detection On Environmantal Pollutant

The capillary electrophoresis (CE) technique is applied in the thesis. According to different types of environmental pollutant, we set up direct UV detection, indirect UV detection and UV-derived detection methods to analyze a series of environmental pollutants such as metal ion pollutant, anion pollutant and marine pollutant.This thesis contains 5 chapters. The first one gives a general introduction of the capillary electrophoresis, and the 2nd chapter investigates the ion pollutant relevant to CE. The 3rd chapter introduces the sea shellfish toxin, which is a typical sea pollutant, then, it classifies some well known shellfish poisons, including DSP, PSP, ASP. NSP and Ciguatera. Finally it introduces their characteristics, poisoning and the distribution respectively, and summarized biochemistry and instrumental analysis methods.The core of the thesis is the 4the one, which contained 5 parts. Firstly, it introduces the analyses of the Cu2+ and Fe3+ carried by direct UV detection, secondly, it introduces the method to measure several cations of Ca2+, Na+, Mg2+ in the tap water. And the 3rd part is about the detection of SO42- in the tap water using indirect UV technique. The 4th part introduces the method to detect Cu2+, Fe3+, Mn2+, Zn2+. The following part concerns about natural organic pollutant as well as its analytical method using CE. Choosing DA(domoic acid) as candidate analyte, we obtained 69 shellfish samples from 53 sites dispersed along seacoast of China (South China Sea, East China Sea and North China Sea). DA was examined not only by CE but also by mass spectra (MS). The result shows that DA was found in shellfishes variously in North China Sea but not found in other areas.Finally, we classified the technological questions while using CE.