| It is well known that Deoxyribonucleic acid (also named DNA) is the transference base of information in the course of life. The destruction of DNA will cause the handicap of life, even discontinuity. So, people focus on the study of DNA. Moreover, cancer is the first killer to cause the destruction of DNA and to threaten human's life. And it becomes the object of the study in the life-science field. In this paper, the Electrochemistry behavior of the anticancer drugs 8-Azaguanine (8-AG) and 6-Mercaptopurine (6-MP) have been studied and the interaction of 8-AG with DNA has been studied too. The main results are expressed as fellows:1: A new oscillographic polarography method for determination of trace anti-cancer drug 8-azaguanine was investigated. In 0.10mol/L H2SO4 solution, a sensitive reduction peak of 8-azaguanine was obtained with peak potential of -1.03V(vs SCE). The peak currents were linear relationship with 8-azaguanine concentrations in the range of 1.00×10-8mol/L~9.00 10-5mol/L with detection limit of 9.00×10-9mol/L. This method was simple operation and high accuracy. The detect recovery was between 95.0 % and 103 %.2: The Electrochemistry behavior of the anticancer drug 8-Azaguanine at wax-marinated graphite electrodes had been studied. In the HAc-NaAc(pH3.60) buffer, one oxidation peak of 8-AG,which was diffuse-driven at graphite electrode, was obtained with the peak potential of 1.20V (vs SCE). The peak current was linear relationship with 8-AG concentration in the rang of 1.00×10-8mol/L~9.00×10-5mol/L. The detected limit was up to 9.00×10-9mol/L.The electrode reaction process had been investigated in detail by lots of electrochemistry techniques, and its dynamics parameters had been obtained. Also, the determined methods for 8-AG, which was sensitive and fast has been performed.3: The interaction of 8-Azaguanine with fish tests double-stranded DNA (dsDNA) and fish tests single-stranded DNA (ssDNA) was studied electrochemically by using differential pulse voltammetry (DPV) and cyclic voltammetry (CV) at wax-marinated graphite electrodes (GE) in a 0.2mol/L acetate buffer (pH3.6). Different effect factors, such as pH, time, temperature, and DNA concentration were investigated. It was observed that the peak current decreased and the peak potential shifted positively with the increasing of time, temperature and DNA concentration respectively. In addition, the peak current was a linear relationship with DNA concentration in the range of 1.00 g/mL ~7.00 g/mL. It was shown that the binding mode between 8-AG and DNA was electro-static interaction. Their electron clouds affected each other and formed a new association of DNA-m8-AG (m=3). Therefore, when 8-AG cures a cancer cell, it damaged a normal cell. Furthermore, The drug 8-AG would not affect the DNA molecule after 90 minutes at room temperature. Simultaneously, the electrode reaction process was investigated in detail by some electrochemistry techniques and its dynamics parameters were obtained.4: The voltrammetry behavior for 6-Mercaptopurine (6-MP) on hanging mercury electrode had been investigated by using some electrochemical methods. In thephosphate buffer solution (pH7.40), 6-MP showed a pair of redox peak controlled by adsorption. The oxidation peak current and the reduction peak current were a fine linear relationship with the concentration of 6-MP in the range of 9.00×10-6mol/L-1.00×10-4mol/L and 4.00×10-5 -1.00×10-4mol/L respectively with the detection limit of 3.00×10-7mol/L and 1.00×10-7mol/L. At the same time, the quantitative analysis method was also used to determine the quantity of the 6-MP in the tablet of Lejining. Finally, the electrode reaction process had been studied particularly and the kinetic parameters had also been obtained too. |
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