| Chitosan, a natural functional biopolysaccharides with excellent biocompatility and no allergic reaction, can be degradated into glucosamine and N-acetyl-glucosamine by lysozyme in vitro. Chitosan has already numerous research and wide applications in many fields such as agriculture, light industry, medicine, bio-medicial materials, environmental protection. Chitosan is obtained by N-deacetylation of chitin. It has a number of free amino group. The content of the amino group will affect the physico-chemical properties of chitosan such as the dissolve ability, the viscosity of the solution, and adsorption properties. At the same time, it will affect the applications of the membranes made by the chitosan in the fields of bio-medical membranes and bio-materials.In this essay, different acetylated chitosan membranes have been prepared through different methods as the cell scaffolds in tissue engineering. The membrane properties including the moisture adsorption, the degradation degree in vitro, the transparency, the crystallization, the permeation and adsorption of bovine serum protein have been studied. The results show that the properties of different acetylated chitosan membranes have little difference. But they all have high moisture adsorption and transparency, a certain extent permeation and adsorption of bovine serum protein, and can be degradated by lysozyme in vitro. The results indicate that chitosan and its acetylated derivatives have potentially broad application in the fields of bio-medical membranes.Keratopathy is a kind of familiar disease, which is an important factor for the blindness in clinic. The culture of corneal cell in vitro is of significance to the corneal biology, pathology, pharmacology and corneatransplantation, and it is also an important part of the reconstruction tissue engineered artificial cornea. In this essay, the culture methods of corneal epithelial cell and keratocytes have been studied. The method of rabbit corneal epithelial cells has been improved in order to solve the problem that the cells moved slowly and could not form monolayer when the traditional tissue nubbles cultivation was adopted. The epithelia are properly digested by trypsin before processed nubble cultivation. Cultured a week later, the corneal cells present polygon and array tightly, and have active proliferation and excellent status, which look like the corneal epithelial cell in vivo. It proves to be an ideal primary culture method of corneal epithelial cell. The keratocytes have been cultured by enzymatic digestion culture method. Cultured a week later, the cells grow rapidly.Two weeks later, the cells can overspread the culture plate, which looks like spindle. After two weeks subculture, the cells can overspread the culture plate, most of which look like shuttle. According to what we have been done above, the primary culture of human corneal epithelial cells and keratocytes has been tested. Cultured ten days later, the human corneal epithelial cells present polygon and array tightly. Cultured fifteen days later, keratocytes present spindle, and grow in bilayer somewhere.Reconstruction of tissue engineered artificial cornea in vitro is an efficient way to solve the shortage of corneal transplantation materials. Chitosan and its acetylated derivatives have been widely applied in the nerve, cartilage, bone tissue engineering. In this essay, the corneal epithelial cells and keratocytes were seeded onto different acetylation chitosan membranes. The cells on the membranes were observed in order to study the biocompatibility between the corneal cells and the membranes. The results show that the epithelial cells can grow well on a certain acetylation degree membranes. Cultured a week later, the epithelial cells present polygon and array tightly. The cells can overspread the whole surface of the membranes, which indicate that theyhave excellent compatibility. After cultured two weeks on the membranes, the keratocytes present spindle, even grow in bilayer somewhere on a certain acetylatio...
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