Investigation On Oscillographic Behavior Of β-Cyclodextrin And Its Application

Oscillographic characteristic of 3 -cyclodextrin and its inclusion action with metal ion, some amino acid and some organic medicament were studied in this thesis. β-cyclodextrin and substance included were determined by second order differential simple Oscillographic voltammetry. This thesis consisted of four chapters, and main contents are as follows:1 . β-Cyclodextrin can caused a reversible incision at -0.38V(vs. SCE) on dE/dt - E curve in 0.2mol/L NaOH, and incision depth on the cathodic branch of dE/dt - E curve was deepen with the increase of β-Cyclodextrin. Based on this oscillgraphic characteristic, a method of second order differential simple oscillgraphic voltammetry for determination of β-Cyclodextrin was founded.2. In 0.2mol/L NaOH, the sensitive incision of P-CD would get shallow with increase of the inclusion complex formed between Cu2+ and p-CD. Based on this oscillgraphic characteristic, a method of second order differential simple oscillgraphic voltammetry for determination of Cu2+ in silicon brass was presented. The linear range for Cu2+ and and the detection limit for Cu2+ is 1.0×10-6-2.0×l0-5mol/L and 8.0×10-7mol/L, respectively. The regressive equation and correlation coefficient are h(v)=-3.861×l04C+4.819(mol/L) and (r=-0.9992), respectively. The RSD for determination of 7.0×10-6mol/L Cu2+ is 2.1%(n=5), and average recovery is 102.4%(n=5). Inclusion ratio of Cu2+ and P-CD was determined by second order differential simple oscillgraphic voltammetry. Compared with other methods, this method has the advantage of cheap equipment, simple operation and no need especial pretreatment for the sample.3. Because of especial molecule structure and oscillgraphic characteristic of β-CD, L-Phenylalanine and L-Tyrosine with Non- oscillographic active substances and Phenobarbital and Rutin with oscillographic active substances can form inclusion complex rapidly with P-CD in 0.2mol/L NaOH, and the peak height on second order differential simple oscillogram of β-CD would decrease. Based on this oscillgraphic characteristic, a second order differential simple oscillographic voltammetry for the determination of L-Phenylalanine, L-Tyrosine, Phenobarbital and Rutin was founded. Inclusion ratio and inclusion constant were determined with equal mol continuous change and Job method, and inclusion mechanism was discussed elementary. The experimental results showed that indirect determination method of inclusion system would be used successfully when the direct determination of medicine is not so sensitive or the medicine has no oscillographic active. As a result, the sensitivity of the determination for the medicine can be improved significantly and the application field of oscillographic analysis is enlarged accordingly.4. Based on the characteristic of the inclusion complex formed between p-CD and the hydrolysate of Amoxicillin in 0.2mol/L NaOH, a second order differential simple oscillographic voltammetry for the indirect determination of Amoxicillin was founded, and with equal mol continuous change and Job method, the inclusion ratio and the inclusion constant determined is 1 : 1 and 6. 0×105, respectively.