| In this paper, silver nanoparticle has been prepared. The relationship between absorption spectra and resonance Rayleigh scattering (RRS) and the size and concentration of silver nanoparticle have been inverstigated. Furthermore, the reaction between silver nanoparticle and some cation dyes such as basic triarymethane dye, basic phenazine dye, basic phenoxazine dye. basic xanthene dye and protein and antibiotics were studied by RRS. The spectral characteristics, the influencing factors, the properties of analytical chemistry and the probability of their analytical application have been investigated. The new, simple and fast methods for the determination of silver nanoparticle, protein and antibiotics have been proposed.1. Study on the relationship between absorbance spectra and resonance Rayleigh scattering spectra and the size and concentration of silver nanoparticle.Liquid phase nanoparticles with different diameters and colors can be prepared with sliver nitrate-sodium citrate reduction method by controlling temperature and reaction time. The mean diameters of sliver nanoparticle are measured by transmission electron microscopy (TEM). They have specific absorption spectra for the absorption peaks and there are bathchromic shifts gradually with the increase of diameters of silver nanoparticles. And there are obvious resonance Rayleigh scattering (RRS), second-order scattering (SOS), frequency-doubling scattering (FDS). The maximum peaks λRRS, λsos, λFDS are located at 470nm, 940nm, 470nm respectively. When the concentration of silver is constant, the scattering intensity IRRS, ISos and IFDS have linear relation with the diameters of silver nanoparticles. And, when the diameter of silver nanoparticle is constant, IRRS, Isos and IFDS are directly proportional to the concentration of silver nanoparticle. Therefore, absorption and RRS, SOS, FDS spectra can be used to study the size and to determine concentration of silver nanoparticle.2.Resonance Rayleigh scattering spectral study of the reaction between triarymethane dye and silver nanoparticle.Our experimental results have shown that, in pH 2.4~2.6 acid medium, when silver nanoparticle reacts with a basic triaminotriarymethane dye such as Crystol Violet, Methyl Violet, Ethyl Violet, Methyl Green or Iodine Green to form a compound, the intensity of RRS enhanced greatly and a new RRS spectrum appears. But the RRS of the basic diaminotriarymethane dye such as Malachite Green, Brillant Green enhances weekly. Because the ration of IAg-cv /IAg is maxium when silver nanoparticle diameter is 40nm, the diameter was selectived in the experiment. And the system of Ag-CV has obvious frequency-doubling scattering (FDS) and second -order scattering (SOS). Among which, RRS is the most sensitive, and the detection limits of Ag is 1.1-1.3 ng/mL. The method which modifies silver nanoparticle chemically provides a sensitive and simple method for the study and detection of silver nanoparticle. The hyerchomic effect of silver nanoparticle and basic triarymethane dye system can be observed. The suitable reaction conditions have been studied in the paper. And the probability of determination trace Crystol Violet in water has been discussed preliminarly using 20ng/mL silver nanoparticle. The method has high sensitivity, but there exist more interfering substance. If the selectivity is improved or the necessary preseparation is complemeted, it can be used to practical analysis.3.Resonance Rayleigh scattering spectral study of the reaction between basic phenazine dye and silver nanoparticle.In pH 2.4-2.6 acid medium, when a silver nanoparticle with diameter of 40nm react with basic phenazine dye such as Phenosafranine Chroma (PC) and Netruel red (NR) to form a compound, the intensity of RRS enhanced greatly and a new RRS spectrum appears, and obvious freguency-doubling scattering (FDS) and second-order scattering (SOS) appears at the same time. Among which, RRS is the most sensitive, and the detection limit of Ag is 0.8ng/mL (PC-Ag) and 0.6ng/mL (N... |
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