| Based on the principle of Hunter reagant,N-Hydroxysuccinimide(NHS) functional group was introduced into polymer carrier to synthesize a couple of olefine acids' and styrene' copolymer-NHS-esters.' Conditions to prepare the NHS-esters of all olefine acids' and polymer-NHS esters were studied. And these products' basic physical and chemical properties were also tested.Self-prepared polymer-NHS-esters were coated on PS test tubes and small PS balls and got a kind of function material which can connect with proteins through covalent bounds. Various conditions of this material to bind proteins were studied: reaction temperature, time, media , protein types and so on . The test results showed that the amount of this kind of function material to conjugate proteins were larger than 1.5[ig/mm2and its one time adsorption rate was larger than 80% and can satisfy the requirements for solid-phase radioimmunoassay. Test results showed that this polymer-NHS-ester can connect with proteins through covalent bounds under mild conditions This technical method will supply a type of new material for solid-phase radioimmunoassay(RIA).The method and effect of using self-prepared solid-phase iodine-activated beads to iodine-label proteins were studied.The effects of various iodine-labeling conditions such as the using dose and size of activated beads ,pH value,temperature,labeling timeperiods ,the volume,and the dose of protein on labeling rate weretested.The results suggested that iodine-labeling can be performed under mild conditions and which has a wide range of applications and its labeling effectiveness is high and the substances labeled were quite stable and it has very little damage to the bio-activity of the labeled proteins.So it has a bright application prospect. |
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