Research Of Charge Transfer Interacting And Application Research Of Fluorescence Spectrophoto-metry In Biochemical And Pharmacutical Analysis

Charge transfer reaction belongs to that kind of reaction in which charge transfer complex is formed by charge transfering between electron donor and electron acceptor. Charge transfer spectrophotometry that is used in pharmaceutical analysis is brief, fast and exact. So it appears to good application foreground. And two kind of medicine are determined by Charge transfer spectrophotometry. One is that the charge transfer interacting of vitriol atropinum with iodine in the chloroform was studied. Iodine interacts with atropinum in the chloroform. The maximum wavelength of the complexation is 288nm and the apparent molar absorptivities of the complexation is 2.66 X104L . mol"1 ?cm-1. The molar combining ration is 1:1 ?The reaction mechanism was studied. The method is simple and convenient, shortcut, exclusive, the linear calibration range is 0-35 Ug/ml, the reclaim ration is 99.6%, RSD is 0.83%. The other is that x -Electron acceptor tetrachlorobenzoquinone can form charge-transfer complexes with Lomefloxacin Hydrochloride. Optimum conditions for determination, linear calibration range and composition of complexes have been reported. The proposed method has been applied to the determination of lomefloxacin Hydrochloride in capsules with satisfactory result. Fluorescence spectrophotometry is a high sensitive and selective analytical method. The technique has been extensively applied in biochemical and pharmaceutical analysis. Fluorescence spectrophotometry application of two aspects in biochemical analysis are summarized from resent years' reasearch. And we have done some work on biochemical and pharmaceutical analysis. The interaction and irradiance of Calix[4]arenes > Praseodymium[III] and DNA is researched. The result of experiment indicates that Praseodymium[III] can produce fluorescence ( exjuax=300.0mrK,-orvmx^OO.Onm). Calix[4]arenes can quench its fluorescence under the certain condition. After DNA is putinto system, fluorescence intensity of system boosts up greatly. So DNA can be determined by using Fluorescence Probe of Irradiance Lanthanides Supermolecular composed by CaJix[4]arenes and Praseod}mium[III]. At the same time, mechanism of interaction among compounds in the system is discussed. The linearity range is 0-1.0 u g/ml and 2.0-4.0 u g/ml, The limit of the detection is 1.276 X 10-3 y g/ml. The method is simple, sensitive and reliable. A method of fluorescence spectrophotometry for study of fluorescence spectrum characteristic and determination of lomefloxacin hydrochloride in serum by florescence spectrophotometry is developed. The linear range of the method is 0.005~5.0ug/ml. The average recovery of lomefloxacin hydrochloride in serum is 99.5%. RSD is 0.82%.