Study On The Improving The Technological Condition Of Bacterium GY3 Fermentation Production PHAs

In according of Bojie Bacterium Determinative Handbook (8th edit) , from its morphological character, physiologic and biochemical reaction character, (G+C)% content, We did strain qualification test and made certain that GY3 belonged to Pseudomonadaceae. Experimentized the shake bottle fermentation optimization and the best supplemented medium fermentation condition was as follow : 2.5% sugar concentration controlled, 10% inoculation amount, C/N=2.5, 3%original sugar concentration, supplemented 0.5% (NH4)2SO4 (8,16,32h), When we use tap water as solvent, Fe2+ and microelement had no effect on cellmass and PHB yield. The total fermentation time was 50 hour and desiccated cellmass was 34g/L, PHB yield was 23.4g/L.Based on shake bottle result, experimented fermentor simple batch and supplemented batch fermentation. The best supplemented medium condition was as follow: 1600ml volume medium, 1:1 aerate measure, variation rate culture and the results was 53.74g/L desiccated cellmass, 45.9g/L PHB yield, 85.4% PHB production ratio. Founded fermentation biochemical kinetic model and made the experiments mount up the academic altitude from experiential fumbled. Through model validated, we confirmed that the model could simulated the truly diversification process of state variable cellmass(X), outcome(P), medium(S), exactly on the whole. Provided thereunder for magnified manufacture.Experimented 15L fermentor supplrmented medium magnified experiment and the results was 52.2g/L desiccated cellmass, 43.4g/L PHB yield, 83.2% PHB production ratio. There is more lucubrate under way.