| Capillary electrophoresis(CE) has been widely used as a powerful separation and analytical technique in recent years because of its advantages including high performance, high speed, low analytes and sample consumptions. It has been applied to a wide range of research fields such as chemistry, pharmaceutical analysis and life science et al. This paper consists of three chapters. In the first chapter, the elementary theory of CE and its application on the pharmaceutical analysis and developing direction were introduced. In the second chapter, a micelle electrokinetic capillary chromatography (MECC) method to separate and determine oleanolic acid and ursolic acid isomers simultaneously in Prune]]a vulgarisL. was developed. The two compounds were baseline separated under the optimum buffer condition of 15 znrnol/L disodium hydrogen phosphate + 15 mmol/L disodium teterborate + 10 mmol/L sodium dodecyl sulphate(SDS) + 5%(v/v) alcohol and their contents in Prunella vulgaris L. with different extracted methods were quantitatively analyzed within 20 nun. In addition, the effects of SDS concentration and organic modifier (alcohol) concentration on the separation of the analytes were also investigated. In the second section of chapter 2, the contents of the isomers in folium photiniae and [los ca.mpsis were determined under the same optimum buffer condition as above. In the third chapter, the formulas for CE determination of pKa and pKa2 were introduced. The pKa and pKa2 values of phenol, hydroqninone, resorcinol, phloroglucinol, pydroxybenzyl alcohol and gastrodin were determined and were consistent with those in literatures. The pK, values were achieved by noninear regression analysis by taking into consideration the effect of activity coefficient completely. This was the first report on determining the dissociation constants of magnolol,honokiol, quercetin, curcumin, diethylsti lbestrol, 4--acetanidophenol,ellgenol and paeonol. |
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