Biosynthesis Of Cinnamamide As Secondary Metabolite Of Actinomycete And Strain Identification

Cinnamamide is an important intermediate for organic synthesis and is widely used in pharmaceutical and fine chemical industries. Moreover, cinnamamide has the pharmacolo-gical and biological activity.A new actinomycete strain was found by screen, which can biosynthesize secondary metabolite cinnamamide The actinomycete strain CGMCC No.4487 was identified by 16S rDNA gene sequence analysis, together with the study of morphological, physiological and biochemical characteristics. The results showed that the strain CGMCC No.4487 belonged to Streptomyces turgidiscabies.Using Placket-Burman experimental design and central composite design of response surface method, the fermentation medium condition in a preliminary study was optimized. The results showed that the content of peptone and pH value were the significant factors for the production of cinnamamide. Cinnamamide's production significantly increases 75%, from 160 mg/L to 280 mg/L after the optimization of fermentation medium. It is the first time to biosynthesize cinnamamide with high yield, which has the potency on the biosynthesis of cinnamamide by industry process.The biosynthesis pathway of cinnamamide as secondary metabolite of strain CGMCC No.4487 was invetigated. Through HPLC, the content change of fermentation process was tracked to verify preliminarily that the biosynthesis pathway of cinnamamide as secondary metabolism of strain CGMCC No.4487 was not the metabolic passway from L-phenylalanine to cinnamic acid reported in the previously literature. This paper determined that the polyketide pathway might be related to biosynthesis of cinnamamide by adding inhibitors of different metabolic pathways.