Signal Processing Of Multi-Channel Capillary Electrophoresis Microchip Based On CCD

Capillary electrophoresis has become an accurate and efficient analysis means, which is widely used in genetic sequencing, epidemic diseases screening, blood and drug screening. With the deeper research, much more samples are needed, which needs a large number of synchronous detection to determine the structure and characteristic of the samples. Therefore, to exploit a high-throughput capillary electrophoresis detection system has become particularly important problem.In this paper, the laser-induced fluorescence means was used to realize multi-channel capillary electrophoresis detection system based on CCD. The design of hardware circuits was based on FPGA chip EP2C8, the CCD driver circuit module and A/D controller module were designed with Verilog HDL on Quartus II software platform. The output signals of CCD were processed by a series of pre-processing circuits and A/D conversion, then the signal data was collected by soft core of FPGA, which can communicate with PC. In the end, the detection curve of data was drawn on PC. There were two parts in the software department of the detection system. One is the soft core of FPGA, which was designed to collect the output signals of CCD and transform the data to PC based on C languages on Nios II platform. The other one is software on PC, which was use to collect the data that was transformed from FPGA, draw the detection curve and save data with good user interface.The multi-channel capillary electrophoresis detection system designed on this paper can detect different intensity fluorescence signal in each channel on a four-channel capillary electrophoresis chip, and the signal curves of four channels can be drawn on PC at the same time. The detection system has great characteristic, such as small size, high-throughput, high efficiency, low power consumption and so on, which was a great help for the detection system miniature and portable.