Genetic Diversity Of Miscanthus Floridulus Devealed By ISSR Markers

Miscanthus floridulus (Lab.) Warb. ex Schum. et Laut is an economically important plant. The study were to evaluate the genetic diversity and genetic differentiation of natural populations and present a scientific framework for the conservation and utilization of M. floridulus.We established the reaction system and amplified procedure suitable for M. floridulus, which was 25μL amplification reactions system containing 10×PCR buffer 2.5μL,1.5 mmol/L Mg2+,100 ng template DNA,200 mmol/L dNTPs,0.75 U Taq DNA polymerase. The optimal amplified procedure was as follows:after a pre-denaturing of 5 min at 94℃,45 cycles were performed with denaturing of 45 s at 94℃, annealing of 45 s due from 50-60℃, extension of 80 s at 72℃, a final extension step of 7 min at 72℃.We estimated the genetic diversity and genetic structure of 12 natural populations in Jiangxi province using ISSR. A relatively high level of genetic diversity was detected in M. floridulus using 81 polymorphic microsatellite locie. The ISSR analyses revealed a high level of genetic diversity at the species level (PPF= 92.05%,Na=1.9205, Ne=1.4941, Hpop=0.2911,I=0.4409); and a low level at the populations level (PPF=43.85%,Na=1.4385, Ne=1.2564, Hpop=0.1534, I=0.2286). The genetic diversity of Jing'an population(JA) was the highest.M. floridulus is a perennial species with a wide distribution; these characteristics could account for the relatively high genetic diversity we observed. Furthermore this species has outcrossing and incrossing breeding system, a situation amenable to high genetic diversity and low levels of genetic differen-tiation among populations (Gst= 0.4669). Divided into 3 groups, using UPGMA and POPGEN. Mantel test evealed no significant correlation between the genetic distance and geographic distance (r= 0.3086).