Research On The Simulation Of Metabolic System And Application Of Systems Theory

Abstract: Based on Lactococcus lactis metabolic pathway, the standardized reaction rate equations of the metabolites could be created automatically, and system model was built. At the situation of assuming parameters, the varied curves of metabolites concentration was computed, the metabolic process of Lactococcus lactis was quantified. The results showed that:1) When the enzyme concentration was enough, the different initial value of glucose didn't influence the time that reaction reached balance and the occurrence of the extremum.2) The revised model could apply to mutative strain, then could provide guidance of selecting strain, the complicacy and blindness of experiments can be reduced.In order to solve the probiem that the experimental data and model can not match in the system level, the single reaction process and the metabolic anaplerosis reaction from the point of metabolic mechanism were researched.The results showed that:1) According to the experimental data, the relationships among the instantaneous values of concentration, the average concentration and the pulse value can be created.2) The formula of concentration relationship and the metabolomics data can be matched, then the threshold idea was determined.3) The formula of concentration relationship and the production data of fermentation can be matched, and then the system model of fermentation process was established.In order to ascertain the interaction among immune indicators of the aquatic animals, to understand well the experiment results of improving the animal immunity, the thought of system theory is proposed to analyze the changes of immune indicators. Research shows that:1) The organism immunity of aquatic animals is a complex system and can be studied systematically and comprehensively, that if we study the changes of immune indicator respectively, the relation among the immune indicators would be dissevered and the information of the interaction among indicators would be missed, and that the research results are not comprehensive or incomplete.2) The concept of phase space, the hyper-volume domain or the occupied space are established and used to study the relationship among the various indicators.3) The concept of equilibrium region is established in this paper, and then we point out that the variations of immune system go through three different stages.4) The problems in current routine experiments are pointed out, that is, some experiments are not complete.5) At the end of the paper, the phenomenon of immune fatigue is discussed and pointed that in fact it may be a new equilibrium state.