The Functional Of Aquaporins Genes In Puccinellia Tenuiflora

This topic from the alkali Lemongrass cDNA cloning got the water hole protein gene, on gene expression of biological information, features, and yeast subcellular localization of transgenic plants modified for resolution.Through constructing cDNA library of Puccinellia,and through the library storage capacity to detect, we get a higher storage capacity cDNA library Puccinellia, which has integrity and representative.Through the PutPIP1 gene analysis of biological information, suggesting that the PutPIP1 gene belongs to the Aquaporin family inherent in the plasma membrane protein PIP1 subdivisions, and has six transmembrane structure.Through the PutPIP1 gene expression in characteristics of PutPIP1 in the wild, chinampoensis organizations organs of transcription characteristics to undertake research, indicating that PutPIP1 in Puccinellia in all parts of the expression in a certain amount,and organized specific gene expression in a certain range.Recombinant plasmids will turn into yeast cells, pYES2-GFP-PutPIP1 and green fluorescent protein mingle with GFP as control and guide the PutPIPl gene expression to prove that the yeast is oriented to the plasma membrane.Under certain conditions, the PutPIP1 gene expression show a enhancing tolerance in Na2CO3 and NaCl, and without enhanced NaHCO3 resistance of salt.