Study On A Method Based On Microfluidic Chip For Cell Metabolic Fingerprinting Visualization

The study focus on developing an new method for cell metabolic fingerprinting analysis. First a PVDF (polyvinylidene difluoride) membrane colorimetric array are constructed utilizing the sensitive detection ability for bio-molecules of porphyrin and other dyes, the membrane colorimetric array has a cross-reaction property for multicomponent substance detecton and shows great color profile response to cell metabolites change. The color profile change of the colorimetric array could greatly translate the cell metabolic fingerprinting and make the cell metabolic fingerprinting visualized. Then the colorimetric method was successfully used for cell metabolites change monitoring and cell discriminant with cell standard culure. The cell standard cell culture method are explored with the conference of Biolog stystem for standard microorganism culture, six kinds of cells are cultured with the cell standard culture and used for colorimetric array detection, and the HCA and PCA analysis show that the colorimetric array cell metabolic fingerprinting method could be used for cell discriminant. Based on the cell culture, fluid control and real-time detection abilities of microfluidic chip, four microfluidic chip are designed for colorimetric array real-time detection, and further used for the microfluidic chip-colorimetric array-CCD contruction. The microsystem integrate on chip cell culture and colorimetric detection on a biochip. The microfluidic chip colorimetric method is simple, miniaturization, harmless, real-time response to cell multicomponents metabolites, and have a high potential for cell research and drug screen.