Construction Of Infertile Mice Model And Phenotype Analysis Of Oocyte-G1 Knowdown Mice

One fundamental characteristic for living organism is its dynamics. In all process of generation and development, living organism undergoes a series of dynamic events, which accurately regulates their physiological behaviors and maintains their body function. Molecular motor is such kind of dynamic protein, which highly couples chemical and mechanical energy, and regulates physical function through molecular movement.Kinesin Oocyte-G1, a protein affiliated to molecular motor, is a new member of Kinesin superfamily, which was firstly discovered in our lab years ago. Previews studies both in vivo and in vitro have revealed its crucial function in the process of reproductive development. In this study, we constructed an infertile recepient mice model and tried to using Oocyte-G1 knockdown mice to depict its physiological function comprehensively in vivo. Here, we report that interfering Oocyte-G1 expression will cause lethal effect at period of adolescence. Dramatic decrease in size and weight was seen in these models. In interfered male mice, testis atrophy was enormously predominant and further histological examination reveals developmental arrest of spermatogenic cells in pachytene stage and inner lumen loss in impaired seminiferous tubules. Phenotype of irregular-shaped oocyte and developmental retardation was displayed in female ovary. Abnormality was also found in dentate gyrus, but not in other organs such as kidney and lung which was also Oocyte-G1 enriched.Thus, our results demonstrated that Oocyte-G1 is essential for mouse development. Depleted expression of Oocyte-G1 will result in dysfunction in many organs, especially those for reproduction. This research provides new data for Oocyte-G1 functional identification, which is significant for further in-depth exploration in molecular mechanism of this new gene and other molecular motor proteins. It also set up a new potential target for cancer therapy development and chemomechanical-coupled artificial molecular motor investigation.