TSA Increased The Rate Of The Blastocysts And Estrus Synchronization Treatment On The Embryo Transferabstract

The experiment lets bovine fetal fibroblasts become the ciliary cell,carries on the somatic cell nucleus transplant by the cow ovocyte as the acceptor butcher nature. Bovine fetal fibroblasts were seeded into DMEM containing 80nmol/L of TSA 12 h,then the nuclear transfer (NT) embryos that constructed with bovine fetal fibroblast cells were seeded into H-M199 (2-3h) and SOF containing 80nmol/Lof TSA for 0h,6h,12h,24h. Laser scanning confocal microscope for somatic cell histone H4K12 acetylation, and detected by nuclear transfer cloning of embryonic development rate at different time. At the same time,we compare the embryonic implantation effect in the method to the different time in order to find suitable local conditions estrus synchronization programs. Uses injection prostaglandin (PG), two injection prostaglandin (PG), the vagina to bury separately plants the hitch (CIDR)+the prostaglandin (PG) three methods to carry on in heat same time processing, and supposes the nature to be in heat group to make the embryonic implantation effect comparison.1,TSA can increase the levels of histone acetylation of H4K12 after bovine fetal fibroblast Cells treated with 80 nmol/L TSA for 12 h(P<0.05). 2,80 nmol/L TSA treatment of nuclear transfer (NT) embryos for 12 h increased blastocyst development compared to controls (21.9%vs16.5%, P<0.05).3,Marked difference existed in blastocyst development of nuclear transfer (NT) embryos which is treated with 80 nmol/L TSA for 24 h and control group (p<0.05); No marked difference existed in blastocyst development of nuclear transfer (NT) embryos treated with 80 nmol/L TSA for 6 h and control group (p<0.05).4,The result of the three groups is similar,double PG injection, CIDR+PG treatment and the single injection of PG.The synchronization rates were 74.70%,77.64%,71.19%,correspondingly the pregnancy rate of fresh embryo was 52.12%,53.26%,50.44%, after embryo transfer, and that of frozen embryos is 46.87%,41.44%,44.00%.5,The recipient utilization of spontaneous estrus was 85.71%, significantly higher than the double PG injection group, the success rate of embryo transfer can be remarkably improved by utilizing recipient of spontaneous estrtus.