Studies On Extraction And Separation Of Sanggenon C

Sanggenon C has a wide variety of pharmacological activities, such as antimicrobial activity and lowering blood pressure. And it can cure the heart disease and atherosclerosis. Sanggenon C has also exhibited significantly inhibitory effect on melanogenesis and anti-oxidantive effect. It can be also used in cosmetic industry.The contents of sanggenon C in extracts from leaves, mulberry and root cortices of mulberry were determined. The result was that the root cortices of mulberry were the best resource. The effect of extraction technology on sanggenon C from root cortices of mulberry was studied with the yield and the purity of the extracts used as evaluating indexes in the dissertation. The best technology was microwave assisted extraction.The effects of ethanol concentration, extraction time, microwave power and the ratio of solvent to solid on the yield and the purity of the extracts were researched in the experiment. Then orthogonal design was used for the research. From the value of variance analysis, the maximal factor that impacts the yield of extracts was the ethanol concentration, while the main factor impacting purity was the ratio of solvent to solid. The optimum extraction process was as follows:the root cortices of mulberry were extracted by 90% ethanol at 60℃for 2 times, each time for 10 minutes. The ratio of solvent to solid is 10:1, and the microwave power is 600W. In this condition, the result shows that the average of yield was 21.60% and the average of purity was 2.53% for repeat experiment.The adsorption and desorption properties of sanggenon C on macroporous resins were compared. AB-8 resin displayed the best adsorption and desorption capacities based on the research results. Both Langmuir and Freundlich isotherms were used to describe the interactions between the solutes and resins at different initial concentrations. Dynamic adsorption and desorption experiments on AB-8 resin packed column were conducted and the optimum parameters for adsorption were as follows:flow rate is 3BV/h, feed concentration is 0.58mg/ml; for desorption:eluent is 6BV ethanol-water (50:50, v/v), flow rate is 6BV/h. After the treatment with stepwise elution on AB-8 resin, the content of sanggenon C in the product increased from 2.53% to 38.87% and the recovery yield was 84.78%.The concentration of 90% and 50% ethanol solution was conducted by nanofiltration and the effects of feed concentration on flux and retention were studied. The results showed that STARMEMTM 122 gave high sanggenon C retention, which indicated that STARMEMTM 122 could be applied to concentrate the 90% and 50% ethanol solution of sanggenon C.The component of 50% ethanol eluant from the AB-8 resin was used as raw material for further purification by preparative HPLC. And the tyrosinase inhibitory activity of products obtained from each step was isolated. The tyrosinase inhibition rate of the component eluted from the AB-8 resin was 97.48% which was better than traditional inhibitor.