Diversity Analysis Of Culturial Bacterial In Uliansuhai

Recently, eutrophication of lakes have become a protrudent environmental problem in the world. Bacteria has the qucikest response rate to the change of surroundings, and the relationship between bacteria community and water eutrophication is still in investigation now. In aquatic ecosystems, bacteria can decompose Organic macromolecules, and translate them into either Bio Energy or the Organic macromolecules which can provide nutrient salt for phytoplankton. So it is generally considered that bacteria play an important role in the transformation of organic matter and the procedure of alimentary cycle. The comprehensive understand to abundance and diversity of aquatic ecosystems will help to the further cognition of lake bacteria's characteristics, and evaluate the contamination degree of lake. This paper researched the structure, abundance and diversity of phyletic evolution of microbial community from extremly severe district-Hong Gebu area in Wu Liangsuhai, in order to improve and develop the correlative theory.This paper aim at the cultivable bacterias from eutrophicated lakes of wuliangsuhai, researched the bacterial diversity of them by the method of adding up the number of culturable planktobacteria, pure cultivation, and phyletic evolution analysis based on the 16S rDNA sequences, with the purpose of revealing the relationship between the abundance, diversity of culturable planktobacterias and the eutrophication of Wu Liangsuhai.The results show that the total number of bacterias of Wuliangsuhai water is 1×105 -6.0×106 cfu/L. The low abundance index indicates that although the lake of Wu Liangsuhai has large number of bacterias, the diversity is low and has low speices equitability. It is likely that the bacterias focus on certain types. Separated 50 bacteria strains from the eutrophicated lakes in Wu Liangsuhai by the Sodium carboxymethyl cellulose(CMC-Na), beef extract peptone, photosynthetic bacteria medium and so on. According to the reviewing of morphological characteristics and partial physiological and biochemical experiments results, selected 41 representative strains as the objects of phyletic evolution analysis based on the 16S rDNA sequences. The results show that these strains belong to Gammaproteobacteria,Firmicutes, Alphaproteobacteria, Actinobacteria, Betaproteobacteria respectively. 27 OUT in all, among which they were highly similar with certain group of known bacteria such as Bacillus, Micrococcus, brevundimonas, Marinibacillus, Pseudomonas, Planococcus, and groups ofγ–proteobacteria become the superiority bacteria, which may be the response to Wu Liangsuhai's eutrophication. In this study, found some Bacillus which have fuctions of eliminating the organic pollutant and posionous matters and the Novosphingobium utilized the Phenanthrene as the only carbon source, both of them can be used in the management of environment, which will lay foudation to develop and utilize the biomass of Wu Liangsuhai and the environmental repair of it.