Roles Of EUI On Regulation Of Development And Stress Response And Zinc Finger Protein WX1 Controlling Of The Flowering Time In Arabidopsis

Partâ… : A previous study of EUI functions in rice has been carried out, Demonstrate that the rice ELONGATED UPPERMOST INTERNODE (EUI) encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice. We have also shown that EUI functions both in the GA metabolism and signaling pathways, GAs metabolism and signal transduction are regulated through complicated networks in the plant, The GA mediated development pathway also actively cross-talks with other hormone mediated pathways in plant development and adapting environmental signals.In order to further study its effects on plant development, we have overexpressed EUI in Arabidopsis. The transgenic Arabidopsis plants (overexpression of EUI) constitutively expressing are extremely dwarfed and decreased seed productivity. Furthermore, The area of the leaf smaller than the wild type, On the cytological level, The transgenic Arabidopsis plants were significantly reduced in the appearance and the cell size compared with the wild type, indicating a negative regulation of EUI in cell division and elongation.Another study found that EUI-OE seeds are sensitive to ABA compared to the wild type in the germination and the capacity of the germination decreased significantly. Growth under various abiotic stress conditions(salt stress, drought stress and heat stress) treatment, The EUI-OE palnts were more insensitive to salt and drought stress than the wild type. On the contrary, The EUI-OE palnts were more sensitive to high temperature than the wild type. All these datas suggested that the content and signal of GA was related to the responses of plant under abiotic stress conditions.Partâ…¡: A mutant which phenotype is later flowering time was gained through Arabidopsis T-DNA mutants screening in the lab. Sequenced analysis revealed that T-DNA insertional mutant is a zinc finger protein named WX1, Which is an unknown function protein, RT-PCR analysis indicated that the mutant is likely true knockout mutant, All transgenic lines displayed early flowering time compared with wild type from our analysis of transgenic, Arabidopsis plants that overexpress WX1 (WX1-OE) driven by the constitutively active cauliflower mosaic virus 35S promoter. These results supported our prediction that WX1 likely function in regulation of flowering time pathway. We transiently expressed WX1-CFP fluorescent protein fusions in onion epidermal cells.The result demonstrated zinc finger protein WX1 mainly located in the nuclear, In the future, We will further research the function of WX1 by genetics and molecular biological methods and revealed the regulation mechanism of WX1 in the flowering time pathway.