Immobilization Of β-galactosidase And The Filtration Of Its Preserver

This paper, mesoporous MCM-41 immobilizedβ-galactosidase, using glutaraldehyde as crosslinking agent and then cross-linkedβ-galactosidase and to improve the stability of the immobilized enzyme,Identified in theβ-galactosidase concentration of 0.5mg/ml , the glutaraldehyde concentration was 4.0%, pH 7.0, immobilization time was 12.0h, the cross-linking time was 120min of the above conditions forβ- galactosidase in the best conditions for immobilization。Through the filterβ-galactosidase immobilized protecting agent, established the lactose toβ-galactosidase immobilization of the best protective agent, can effectively andβ-galactosidase in combination to protect their active sites at a fixed the process of being attacked. galactose can be used asβ-galactosidase immobilized protecting agent , Increasing the concentration of the product from the inhibition ofβ-galactosidase active site exposed to the direction of change, also to protect the purpose of its active site。...