Effect Of 0.2mT Power Frequency Magnetic Field On The Participating Of Ca²⁺ Of Microfilament Cytoskeleton Marginalization Of Fibroblasts

50Hz PFMF (power frequency magnetic field) is the common magnetic field in our environment. Our previous investigations have indicated that:exposure of 0.4mT 50Hz PFMF for 30 min to CHL cells induced EGFR clustered of the cell surface, and this effect can be inhibited by the tyrosine kinase inhibitor PD153035, this PFMF also induced cytoskeleton reorganization of the cells. More evidence showed that an even weaker PFMF (0.2mT 50Hz) also induced cytoskeleton reorganization of FL cells. Decreasing [Ca2+]。partly blocked this phenomenon. EGFR and F-actin are associated closely in cells. Ca2+ places an important role in the EGFR signal transduction pathway. Theraflu, PFMF may influent the EGFR signal transduction pathway and Ca2+ signal transduction pathway.In order to illustrate the role of Ca2+ in the progress of cytoskeleton reorganization by PFMF, in this work, by using techniques as PCS, confocal microscope, flowcytometry and FL cell line we studied the bioeffect of 0.2mT, 50Hz PFMF to understand the mechanism of PFMF interact with the signal pathways such as the cytoskeleton reorganization and Ca2+ signaling.The results obtained from the research are:under the exposure EGFR proteins or FL cells to 0.2mT 50Hz PFMF for 30 min, (1) EGFR proteins clustered after the exposure by using PCS, (2) the amount of cell skeleton F-actin filament decreases compared with the sham in the Flowcytometry experiments, by using the same technique, we also found:(3) the [Ca2+]i of FL cells increased, (4) after decreasing [Ca2+]。or blocking L -Ca2+ pathway, the field-induced cytoskeleton reorganization was greatly inhibited.The results indicated that PFMF induced cytoskeleton reorganization of FL cells is related closely to EGFR clustered and the cellular Ca2+ signaling pathway.