Enzymatic Preparation Of Polysaccharide Fibers And Hydrolyzate Of Cellulose

Corn cortex, which is a byproduct in corn starch production, contains a great deal of dietary fiber and a low content of phytic acid. What's more, It is easy to improve the properties of corn cortex through chemical or biological methods. So corn cortex is a kind of very good raw material to process the dietary fiber (DF).In this study, microbial enzyme method and chemical method were adopted to remove starch and protein existed in corn cortex separately. The results showed that the microbial enzyme method has many advantages in the moderate conditions, the maintenance of natural structure of fiber and a strong specialty, and the active contents such as soluble fiber and hemi-cellulose can be protected effectively to improve the physiological activity of the DF. On the contrary, the chemical methods lead to a drastic removal of starch and protein but a poor selectivity on substrates, and the hemi-cellulose in corn cortex could dissolve in an alkali solution, which makes soluble fiber and hemi-cellulose having important physiological activities lost. From the results of the test, we chose the best technology for the test is:Protease reaction temperature of 50℃for 3 hours enzyme was 1000u / g, boiled 40min after the reaction.So the corn cortex DF obtained from the above is decomposed by xylanase and cellulase in order to get soluble fiber polysaccharides. The product analysis showed that the xylanase dosage 14IU / g, Cellulase dosage 4.0IU / g, reaction temperature 50℃, reaction time 2.5h, substrate concentration of 3%, then the total conversion rate of 60% and Almost no single sugar production, all we need into polysaccharides and oligosaccharides. Through this process, we get the molecular weight between 31560 ~ 136960D soluble polysaccharide hemicellulose and cellulose.