| Objective:To investigate the effects and its mechanisms of Jilin wild pecan oil on apoptosis of hippocampal cells.Methods:The model rats were established by ovariectomy. Experimental groups were respectively given different doses of Jilin wild pecan oil (100mg/kg,200mg/kg, 400 mg/kg) per day.Serum estradiol (E2) levels was detected through Radioimmunoassay (RIA); total serum superoxide dismutase (T-SOD), malondialdehyde (MDA) level were determined by biochemical methods; the expression of anti-apoptotic protein B cell lymphoma leukemia-2 (B cell lymphoma/lewkmia-2, Bcl-2) and Bcl-2 related proteins (Bcl-2 Assaciated X protein, Bax) was observed by immunohistochemistry in hippocampus; Aβcontent in the hippocampus homogenate was measured by radioimmunoassay; PS-1 and ApoE expression in hippocampus were detected by RT-PCR; ultrastructural organization of hippocampal cells was observed by Electron microscope; the apoptosis of hippocampal cells was detected by Hoechst33258 staining.Results:1. The T-SOD activity was significantly enhanced and MDA contents was significantly decreased in Serum by Jilin wild pecan oil.2. The expression of Bcl-2 was enhanced and the expression of Bax was decreased in all Jilin wild pecan oil groups.3. The expression of PS-1 was decreased and the expression of ApoE was enhanced by Jilin wild pecan oil; moreover, the content of Aβin hippocampus homogenate was decreased by Jilin wild pecan oil.4. The apoptosis of hippocampus cells was decreased significantly in all Jilin wild pecan oil groups.5. Compared with the control group, hippocampal cell morphology became normal in large doses of Jilin wild pecan oil.Conclusions:The Jilin wild pecan oil can decrease the apoptosis of hippocampal cells and can protect the hippocampal cells in ovariectomized rats. Its mechanisms maybe concerned with enhancing of antioxidant capacity, modulation of Bcl-2/Bax expression, and decreasing of Aβdeposition in the hippocampal cells via alteration in gene expression of PS-1 and ApoE.
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