The Spatio-temporal Distribution Research On AM Fungi Of 3 Kinds Of Legume Plants In Desert Areas

In desert areas, there live lots of legume plants. Their emergence improved the living conditions for colonization of other plants. Despite their own physiological characters, their endurance capability for the atrocious circumstance also rest with their symbiosis with edaphon, especially the AM fungi. Oxytropis Aciphylla Ledeb, Ammopiptanthus mongolicus Chen f. and Glycyrrhiza rualensis Fisch. not only are the dominant species in desert areas, and have a higher economic value and ecological value. In this study, the symbiotic relationship of the desert legume plants and the AM fungi were used as an entry point,the community composition,the spatio-temporal distribution and their relations to soil factors were investigated and analyzed. Wish can offer the material and basis to degenerate ecosystem restoration and there construction provides.Main research results are as follows1 An abundant species of AM fungi was found in the rhizosphere of the three kinds of legume plants.Isolation and purification of the mycorrhizal fungi led to the identification of 25 species belonged to 3 genera:13 to the Glomus,9 to the Acaulospora and 3 to the Scutellospora.2 Ecological distribution of the diverse species is different:G.dolichosporum and Scu.nigra only occurred in the rhizosphere of Oxytropis Aciphylla;G.reticulatum and A.gerdemannii only appeared in the rhizosphere of Ammopiptanthus mongolicus; A. undulata and A.mellea only occurred in the rhizosphere of Glycyrrhiza rualensis.3 The average colonization of Oxytropis Aciphylla is up to 86.98%, and the average spore density reach 836/100g soil; The average colonization of Ammopiptanthus mongolicus is up to 74.90%,and the average spore density reach 928/100g soil;The average colonization of Glycyrrhiza rualensis is up to 71.78%,and the average spore density reach 1718/100g soil. Showed that the three kinds of legume plants can form well symbionts with AM fungi.4 A distinct temporal variation of AM fungi and colonization of arbuscular mycorrhiza from the rhizosphere of the three kinds of legume plants was found. The greatest colonization and spore density levels were found in the first three layers,between 0～30cm.5 Seasonal variation of AM fungi and colonization of arbuscular mycorrhiza from the rhizosphere of the three kinds of legume plants.The higher spore density existed in summer and autumn.The colonizations of AM fungi are different between plant species.The greatest spore density of Oxytropis Aciphylla was found in October, and the highest hyphal colonization levels was found in May; The greatest spore density of Ammopiptanthus mongolicus was found in August,and the highest hyphal colonization levels was found in October; The greatest spore density and the highest hyphal colonization levels of Glycyrrhiza rualensis were found in August.6 AM fungi and soil have a high correlationship. The colonization of AM fungi and the spore density had a significantly correlated positively with available N, and the others are difference between plant species.This may be related to characteristics of nitrogen-fixation by legume plants,and may be a better countermeasure to adapt to the desert enviroment. Suggest that diverse colonization of different sturctures of AM fungi and the spore density can be used as effective evaluations for sandland soil enviroment.