Taxonomic Research Of Marine Anaerobic Active Bacteria Based On 16S RDNA Level

Taxonomic research about anaerobic activity of strains of marine, not only helps us to explore the marine environment of the resources, expands the types of new species and quantity, but also is the necessary condition about the study of marine anaerobe antibiotics.There is variety of marine microorganisms in the ocean. Because of the diversity of the living environment, such as light, oxygen, pressure and other factors, marine microorganisms have the different morphological structure and physiological functions. And about 80% are anaerobic ones, such as photosynthetic bacteria, sulfate-reducing bacteria, denitrifying bacteria, methane-producing bacteria, etc. The enzymatic system of metabolism is big different between marine aerobic microorganisms and anaerobic ones. In theory, the secondary metabolites will be a greater difference which produced by different metabolism and different respiratory processes. Therefore the taxonomy research of the marine anaerobic microorganisms has the vital significance to the new antibiotic's screening and the new medicine's development.In this paper, 29 strains of marine anaerobic bacteria were isolated from samples, including 9 strains of marine photosynthetic bacteria, 16 strains of marine denitrifying bacteria, 4 strains of the marine manganese-oxidizing bacteria. 5 of them had been identified by 16S rDNA. Results show that the five strains have significant differences in morphological characters. The marine bacterium DN7 was identified as Pseudomonas stutzeri which has the obvious inhibition to Bacillus subtilis and Pseudomonas aeruginosa. There is only 97% similarity between marine bacterium DN3-1's 16S rDNA and others in GenBank, it has been identified as a new species of Alteromonas.