| Mechanism of bamboo flowering is still a mystery. The main reason lies in the specificity of bamboo with complex phenomenon of flowering and long growth course(60~120 years). Experts and scholars hold different theories about the reasons of bamboo's flowering.However, both of them are still in the stage of hypothesis, and still lack of sufficient evidence. It is difficult to explain clearly. Because of the reasons referred above, the main study of bamboo flowering focus on items such as historical data of flowering, flowering phenomenon of bamboo, flowering of the observation and investigation. Phyllostachys bissetii is the staple food of panda. The cycle of bamboo flowering is always the focus of panda's conservation biology, and the core issue of the physiological and biological characteristics study of Phyllostachys bissetii.To carry out the mechanism of bamboo flowering from the molecular level is becoming the new hot spot in recent years. Tian Bo (2005) cloned a MADS-box gene family which is a subfamily of AGL6 flowering gene, from vitro-induced flowering of bamboo (Dendrocalamus latiflorus) , suggesting that the gene may be involved in regulating flowering time latiflorus. This is the first report about the study of the bamboo flowering gene function, which has important significance to understand the molecular mechanism of bamboo flowering.MADS-box gene is a special controlling gene family with unregular gene order. The protein transcription factor encoded by MADS-box gene plays an important role in the growth and development of plants. This gene family in plants (especially in angiosperms) formulated a multi-gene family during the evolution of a large number of gene duplication events.Different members of MADS-box gene family play important roles in the process of plant growth , regulating flowering time, deciding to spend meristem and floral organ characteristics, controling development of root, leaf, ovule and fruit included. Study of MADS-box genes' regulatory sequence can reveal the impact of internal and external factors affect temporal and spatial of gene expression deeply. Research on the MADS-box gene family members' sequence structure, expression pattern and function of proteins coded by these genes, can provide sufficient experimental prooves for the fate of these cognate genes.White niitakayamensis nidularia (Phyllostachys bissetii) will be used as the material of this experiment. We planned to Clone gene of flowering niitakayamensis in molecular level, to study the differences between the expression of bamboo flowering and non-flowering genes. Then we would clone the characteristics of bamboo flowering fragment and describe the bamboo flowering mechanism which would offer some preliminary hints from bamboo's flowering.A cDNA , named PbAP1MADS1, containing the complete coding region and 3'UTR, had been cloned from niitakayamensis'new leaves by 3'RACE and 5'RACE methods. The length of PbAP1MADS1 is 1157bp. PbAP1MADS1, encoding 343 amino acids, has the typical structure of plant MADS-box genes and it is compsed with MADS area, I zone, K and C-terminal district. The results of similarity analysis showed that the subfamily of PbAP1MADS1 belongs to API.And its nucleic acid sequence has the highest homology with the MADS-box protein (Mads1) mRNA of Dendrocalamus latiflorus, with sequence consistency of 92%, and the next is the Oryza sativa (japonica cultivar-group) Os03g0752800with sequence consistency of 85%. The amino acid order of PbAP1MADS1 had the highest sepuence consistency with MADS gene of Dendrocalamus latifloru。...
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