Isolation And Characterization Of A Heavy-metal-resistant Fungi

With the development of industry, the separation of heavy metal ions in wastewater caused serious environmental pollution. It is a tough problem to find a scientific, reasonable and economical method to solve this problem. Recently, bioremediation technology became a promising, green way for this problem. Bioremediation can be defined as any process that uses microorganisms, fungi, green plants or their enzymes to return the natural environment altered by contaminants to its original condition. Compared with the traditional way, there are a number of cost/efficiency advantages to bioremediation.Three strains of epiphyte which can resist heavy metallic ion was separated from the soil of Longmen hill town, Pengzhou city, Sichuan province. In this paper, one strain (Y2) was studied, which is of heavy metal resistance. It is belonged to Penicillium sp, supporting by morphological characteristics and sequence analysis (18S rDNA) .The Y2 tolerated 300 mmol L-1 of Cu2+, and grew on PDA plate ,as for other metal salts, the minimum concentrations (mmol L–1) required to inhibit the Y2 growth on PDA plate were in order of Zn2+,600;Cd2+,<20;Cr3+,<3;Ni2+,<20;Pb2+,<50.The results of orthogonal test in the PDA with three metallic ion:the toxicity of metallic ion are cooperative; the cooperativity depended on the category and concentration of metallic ion. Y2 strain had the ability to resist the three mixture metallic ion. Y2 can adsorb Cu2+ in soil, which has been proved by atomic absorption spectroscopy.