Preliminary Study On The Bacterial Diversity In The Sediments Of Western Area In South China Sea

In this research,we improved the pure culture technique,and the denaturing gradient gel electropherosis technique was applied to study the bacterial diversity variation of the enriched samples from the sediments of western area in South China Sea to evaluate the improved pure culture technique.In the same time,we extracted the 16S rDNA of strains puried by solid media,after cloning,sequencing,the phylogenetic relationship of the bacteria was analysed.The main results were as follows:1.140 bacterial strains was isolated from the sediments of western area in South China Sea by 7 kinds of solid media(NB,DNB,R₂A,DR₂A,2216E,D2216E,LB).2.16S rDNA was extracted from the enrichment samples cultured with 7 kinds of solid media were sequenced.The results revealed that the 14 bacterial strains was from Bacillus,Micrococcus,Halobacillus and Stenotrophomonas.3.After extraction of DNA from enrichment samples cultured in liquid media, the V3 region of 16S rRNA gene was amplified using the primers(GC-341F and 517R).The PCR products,about 200 bp were separated by DGGE..Analysis of the DGGE profiles showed the information of bacteria diversity of the enrichment samples cultured in different conditions.4.The results of physiology biochemistry experiment showed that most of 17 strains could produce kinds of enzyme.So,these strains perhaps have some industrial values.