| Glucosinolates-myrosinase is an important self-defense system in cruciferous plants, which can be referred to as"the mustard oil bomb". When a plant is damaged by pests, it may release myrosinase to degrade glucosinolates and generate lots of biologically active substances. This paper deals mainly with the role of myrosinase gene playing in the relationship between plants and insects based on the RNAi myrosinase gene of Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee plants. The regeneration system of Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee was developed in favor of Brassica regeneration in MS culture medium mixed with 6-BA 5 mg/L, NAA 0.2 mg/L, AgNO3 4 mg/L. In the meanwhile, 16 resistant calli and 5 resistant regenerated plants were obtained based on the expression vector of RNAi myrosinase gene. Molecular detection confirmed that the 5 resistant regenerated plants were all transgenic plants, whose mRNA expression of myrosinase gene had been significantly inhibited in terms of RT-PCR detection. Volatiles released from undamaged and damaged plants were analyzed and compared between normal and transgenic plants of Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee, it was found that there existed more alkanes and alcohols in the normal plants, while more terpenoid, aromatic and heterocyclic volatiles in the transgenic plants. The larval and pupal weight was higher, eggs laid more, and duration of the larval stage shorter when diamondback moth (DBM), was fed with normal plants compared to DBM fed with transgenic plants of Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee, which implies that the normal plants of Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee given well expression of myrosinase gene were more attractive to DBM, and RNAi plants might be used in developing integrated DBM management program.
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