Enhance Of The Oil Content In Mortierella And Preliminary Study Of The Preparation Of Microorganism Diesel Oil

For the past few years, peoples demand of oil especially the functional oil rapidly ascend.Meanwhile, petroleum exhausted in short order, and plant oil cannot meet the increasingly need of society because of the influence of manufacturing location and climate. So microorganism oil are payed attention to by researcher from many countries for it's particular dominant.This artical product microorganism oil by fermentation of Mortierella, investigated three details below:1. inspected inlfulence of temperature, inoculation, ventilate, pH on oil produnct in the process of fermentation. Definite that the fermentate temperature is 28℃in the preceeding time and 20℃in the later period i.e. the 144th hours for ong day. Inoculation 5%, dissolved oxygen 20%, pH5.0～6.0, and in this condition, oil content 46.31%. fundamentally, through add different activations into the ferment fluid, screened out screen out two activation (sodium propionate and glycyrrhizic acid) that promoted the oil content obviously, and the oil content reached 60.12% and 73.13% seperatively.2. Soon extract oil in Mortierella by supercritical CO₂ equipment, surveyed the influence on extraction yield of four factor partical size of raw material,temprature, pressure, time. Find that the best extraction condition is 40℃, 20Mpa, 120min, 20～40 item, and in this condition, the oil content is 43.08%, gas chromatography analysis outcomes show that hexadecanoic acid account for 24.2%, oleic acid account for 54.2%, linoleic acid reached 11.3%,γ-linolenic acid account for 2.8% in the oil.3. further refined the oil, probed the process that preparation bio-diesel by microorganism oil preliminary. Supplyed scientific basis for producting biosel by fermentation of Mortierella.