Effects Of Exterior Stimulators On Camptothecin Induction In Leaves Of Camptotheca Acuminate And Effects Of Heavy Metals On Growth Of Phyotalacca Acinosa

This dissertation was divided into two parts.The first part is the study of effects of exterior stimulators on camptothecin content in leaves of camptotheca acuminate.Through exposing L-tryptophan,salicylic acid,H₂O₂,Cu²⁺ and UV light(UV-C) on young leaves of camptotheca acuminate,the effects of the treatment on camptothecin content were investigated.Some important results are as follows:1.Without UV light,after 2～4 d treatment with L-tryptophan,or with UV light,after 4 d treatment with H₂O₂,Cu²⁺,a considerable increase of camptothecin content was found in the leaves.Among them,the treatment with L-tryptophan or UV light and H₂O₂ were more notably.The contents of camptothecin were 93.1%and 63%more than the control, respectively.2.L-Tryptophan and H₂O₂ could induce the camptothecin accumulation in living young leaves after 2～11 d,the contents of camptothecin were 77.5%,67.4%and 30.8%more than the control,respectively.3.Treatment with salicylic acid,the content of camptothecin was decreased after 2 d,but increased 28.2%after 4 d comparing with control.4.The content of camptothecin in the leaves was decreased during drying process.5.The best concentrations of L-tryptophan and H₂O₂ which could produce the highest increasing of camptothecin content were 20 mg·L^-1 and 2 mg·L^-1,respectively.The second part is the study of effects of heavy metals on the growth of Phyotalacca acinosa.The results were summarized as follows.The growth of the plants were promoted by treatment with low concentration of the heavy metals,while the inhibitive effects were observed when treatment with high concentration.