The Distritution Of HistoneH2A Variants In Mouse PGCs

Specification of prmordial germ cells(PGCs) fate is an essential event during the life cycle of the organism,which segregates PGCs that will eventually generate the totipotent state,from somatic lineages that progress toward terminally differentiated states.Studies on the early mouse PGCs have demonstrated the importance of epigenetic reprogramming including erasure and re-establishment of parental imprints.There has been an increased focus on the role of histone H2A variants in epigenesis.In this study,immunofluorescent staining were used for studying the distritution of histone variants in tissue,single PGCs and cultured PGCs.1 Distritution of histone variants in fresh PGCsDissect the tissues contain PGCs of 8.5～13.5dpc mouse embryo and single PGCs using for immunofluorescent staining.The distritution of macroH2A changed while PGCs enter into Genital ridge(10.5dpc) and after sex distinct(13.5dpc);The change of H2A.X distritution occurred after the sex of PGCs is distinct(13.5dpc);The distritution of macroH2A changed while PGCs enter into Genital ridge(10.5dpc) and fore-and-aft sex distinct(12.5dpc and 13.5dpc).The result showed that the three variants of histone H2A must be concerned with the development of PGCs.2 Distritution of histone variants in cultured PGCsSeparate PGCs of 8.5～13.5dpc mouse embryo and culture them for lday and two day. Immunofluorescent staining were used for studying the distritution of histone variants in cultured PGCs.It reveal that the distritution of macroH2A in cultured PGCs slower than the corresponding period PGCs developed in vivo,H2A.Z in cultured PGCS showed in gear; H2A.X disappear in 8.5dpc cultured PGCs,however it is normal in 10.5dpc～13.5dpc cultured PGCs.Furthermore,the H2A.X distritution in cultured 11.5dpc PGCs reveal that PGCs distinct may had destined before 11.5dpc.