Stress Analysis Of Silica Membrane Under Uniaxial Loading Condition And Influence Of Static Straining To The Morphology Of ECV-304

Substrate loading device is a common cell loading style now.It includes three styles: uniaxial loading, equi-biaxial loading and four-point-bend loading and these styles of loading device all can be set the certain loading frequency. How the cells which adhere to the substrate material response to the stress? And how the each parts of substrate strain? Some papers reported it. However the study of the uniaxial loading is still few. Studying the stress and displacement laws of uniaxial loading process can provide important information to the cell experiment of uniaxial loading.This study had designed a set of uniaxial loading device at first. It can impose the certain straining on the culturing cell. The strain value and style of loading(static straining, static relaxing and cyclical straining)are adjustable. It has excellent aseptic environment and operational stability so can be allied to large-area cell culturing. It can meet the require of the kinds of bio-chemical indicator inspecting.We derived a mathematic model of stress distribution. It can be applied to qualitative analysis though it has a certain degree of error. We had built a finite element model by finite element analysis software ABAQUS 6.7 in the quantitative analysis and got the accurate results of von Mises stress and displacement distribution. It suggests that: the maximum stress value always appeared in the two ends of constraint boundaries, and the minimum stress value appeared in the middle area of the constraint boundaries. With the increasing of the straining rate, the central area appeared and expanded gradually, and the transitional area surrounded the central area finally. The area of edge area had no significant changes in the increasing process of straining rate. The change law of displacement is similar. It suggests that the displacement increasing gradually and gets the maximum value at the edge of mobile clip in the process of the moving from fixed clip to the mobile clip. Displacement is a gradual changing process. Equivalent areas are rectangular and are vertical with the long axis of the silica membrane.The results of cell experiment suggest that: we imposed 10% of static straining on the ECV-304 which was adhered to the silica membrane. Compared the long axis,short axis and ratio of them of static cell and the cell by static straining we found that the long axis of cell 15 minutes after static straining got longer, the short axis got shorter. They had significant difference by T-test(0.05