Sduties On The Modification Of Elastin Via Layer-By-Layer Self-Assembly

Based on the good properties of elastin and the series advantages of layer-by-layer self-assembly technology in the surface modification of biomaterials, in this paper, first of all, elastin were modified via layer-by-layer self-assembly of hyaluronic acid(HA)/collagen(typeⅠ) and the hyaluronic acid(HA)/chitosan(CS) respectively. Secondly, elastin was degraded partly and prepared into 3D scaffolds, and the modifi- cation of the elastin scaffolds was also explored.1. HA/collagen multilayer films were successfully constructed on the elastin membranes. SEM images showed it was clear sandwich structures on an edge of the elastin membranes containing 14 layers of HA and collagen. The XPS analysis demonstrated that the assembly films were HA and collagen. The UV-visible spectroscopy results demonstrated the linearity of the multilayer build-up. The advancing contact angle data revealed the hydrophilicity of the elastin membranes was greatly improved after assembly.The tensile test data showed the mechanical properties of elastin membranes had not changed after the modification.2. Elastin membranes were successfully modified via layer-by-layer self-assembly of HA and CS. SEM images showed the surface roughness of elastin membranes gradually decreased with the number of the multilayer's increasing. The XPS analysis demonstrated that the assembly films were HA and CS. The advancing contact angle data revealed the hydrophilicity of the elastin membranes was greatly improved after assembly. The tensile test data showed the mechanical properties of elastin membranes had not changed after the modification.3. Elastin 3D scaffolds with good properties were successfully prepared. The pore diameter of the scaffolds prepared after 2.5 h degraded in the 0.15mol/L NaOH solution reached 100μm and the pores are absolutely open. The porosity are 76.2% and the tensile strength are 112.6 KPa. The pore diameter of the scaffolds prepared after 4 h degraded in the 0.3 mol/L oxalic acid solution are in the range of 80-100μm and the pores are absolutely open. The porosity are 83.7% and the tensile strength are 81.5 KPa. Furthermore, the elastin scaffolds were modified via layer-by-layer self-assembly of HA/collagen and HA/CS, and the modification improved the bibulous ratio of the scaffolds.