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Study On Biochemical Structure Of Algaes And Its Representations

Posted on:2009-01-03Degree:MasterType:Thesis
Country:ChinaCandidate:S F WangFull Text:PDF
GTID:2120360272475617Subject:Physical chemistry
Abstract/Summary:PDF Full Text Request
All activities of organism, such as growth, development, division and reproduction need energy. Energy is the basis for the existence of life. Especially the algaes. There are two main energy sources which maintain the activities of life: one is light energy(solar energy), the another is chemical energy. But the two kind of energy can not be utilized by organism directly. Light energy need to be transformed into ATP by photosynthesis (photophosphorylase), while chemical energy need to be transformed into ATP by bio-oxidation(oxidative phosphorylation). Then ATP releases the energy which stored in the high-energy phosphate bond by reaction of phosphorylation and hydrolysis, and provides the energy to organisms, while the ATP transform into ADP (AMP). Then ADP can be turn into ATP over again by photophosphorylase and oxidative phosphorylation. So the transformation between ATP and ADP are the tie which connects the solar energy and chemical energy and the basical mechanism which solar energy or chemical energy can be used by organisms. Some research have indicated that the main factors which influence the synthesis of ATP are temperature, pH value of solution, time and intensity of sunlight and concentration of phosphoric acid. And these factors happened to be the main factors which influence the phenomena of"Water Blooms".As important substances——ATP/ADP that supply energy for organisms, their rate of circulation can reflects the fluctuation of metabolism in organisms'cells from some point of view. So study on the structural character and research on the reasons and trend of change about potential energy in the process of energy—transfer from point of microcosmic view can help us understand macrocosmic phenomenon such as development and propagation of algae from the molecule lay. More, it offers new field of vision for studying organism'activities.This text adopts NMR, IR and UV technology to test ADPNa2 and ATPNa2 and compare their results, then analyse the experimental results with VB and MO theory. Based on principle of NMR, experiential formulae of electronegativity and bond energy, We establish function relationship between chemical shifts and chemical bond energy. Then We prove the chemical ideology steadily that structure decides properties, properties reflect structure, there is an important relationship between them. On the premise of distribution of charges in ATP, We expatiate the relationship among chemical shifts, distribution of charges and chemical bond energy, and bring out the qualitative and quantitative relationship further. So that we link remodeling of structure and energy change over again. In addition, we apply the distribution of charges and Electrostatic Repulsion Theory to analyze the process of hydrolysis, and point out the inevitability of bond-breaking position .At the same time, we expound the process of hydrolysis from factors of resonance bond and confusion by Thermodynamics Principle and Resonance Theory.Known as all above, ATP is an important energy-carried molecule in organisms, because it has relationship not only with the special way of combining bond, but also with the charge distribution of atoms. The battlefront orbit determines that ATP have higher potential energy and activity, tends to hydrolyze, releases inner free enthalpy stored in molecules and transforms into ADP and Pi with lower potential energy and bigger entropy, then supply energy for organisms. Due to the particularity of function and configuration, ATP is called"currency of energy"of organisms by scientist. While ATP—ADP circulation also becomes the crucial way of exchange energy in organisms. As a significant testing technique for studying biological molecules, 31P NMR can not only describe space structure of ATP and ADP accurately, but also reveal the chemical principle which substances have followed in the process of energy-transform from the microcosmic view. Furthermore, a new research method was proposed to research the inherent relationship among microcosmic structure remodel, biochemical activity change, energy transfer and thermodynamics macrocosmic behaviors in the process of metabolism.
Keywords/Search Tags:ATP—ADP Cycle, NMR, Valence Bond Theory, Charge Distribution, Resonance Theory
PDF Full Text Request
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