Preparation Of Sialyloligosaccharide-chitosan Polymer And Study On Its Bioactivity

Sialic acid, a nine-carbon monosaccharide derivative, could be combined to form sialyloligosaccharide with oligose of different molecular weights in biosystem. Sialyloligosaccharide was found on the non-disoxidation of glycoconjugates, which was contributed to creating a negative charge molecule and wa s a binding site of IV and other virus. Chitosan was an unique alkalinity polycose in natural world and had many biological activities. But the defect of its poor dissolubility confined the application in industry, agriculture, medicine and so on. The bonding of chitosan and sialic acid solve dissolubility of chitosan could solve the water-solubility of chitosan and educe synergistic effect of them, which was of significance to the study and exploitation of new antiviral drug.1. The Sialyloligosaccharide-chitosan polymer was prepared by chitosan principal chain and sialyloligosaccharide side chain. A new convenient method was used to purify generous sialyloligosaccharide from bovine coloctrum. Through the acid-base reaction between sialyloligosaccharide and chitosan, hydrosoluble sialyloligosaccharide-chitosan polymer was synthesized in the acid condition. The weight proportion between chitosan and sialyloligosaccharide was 3:50, the optimized temperature was 25℃and the time of reaction was 48 hours. The grafting rate, graft efficiency and yield of sialyloligosaccharide-chitosan polymer were 50%, 3% and 8.5% respectively.2. The method of steel pipe was deployed to extraorgan bacteriostasis experiment. The result : The compound of sialyloligosaccharide and chitosan was of aniso- level fungistasis to inaurate, Streptococcus agalactiae, Streptococcus dysgalactiae, aeruginosus Bacillus, sramana bacteria of fowl and Bacillus coli of fowl, but was not fungistasis to bacillus coli of pig.3. The hemagglutination inhibition (HI) was deployed to avian-influenza virus. The result: The effect was the best that the sample two reacted with H5 hypotype and the sample one reacted with H7 and H9 hypotype avian-influenza virus.4.Discarding free radicle, the effect to T and B achroacyte and macrophage and the effect to IL-2, IL-6 were deployed to immunifaction test. The results: The distinction of inhibiting hydroxy radical, boosting SOD and cutting down MDA was predominance between immune suppression mouse and control group when the dosage of sialyloligosaccharide-chitosan polymer was 3 mg/kg; the distinction of inhibiting hydroxy radical and boosting SOD was predominance between normo-mouse and control group, the distinction of stepping down MDA was max-predominance between normo-mouse and control group. The distinction of increment of T,B achroacyte was predominance ; the distinction of englobement macrophage was max-predominance between normo-mouse and control group at 3 mg/kg. The distinction of IL-2 in blood-serum was max-predominance; The distinction of IL-6 in blood-serum was predominance between normo-mouse and control group at 3 mg/kg.Conclusion: The water-solubility of sialyloligosaccharide-chitosan polymer was good and there were more enhancement properties in immunifaction, bacteriostasis and resisting avian-influenza virus than sialyloligosaccharide and chitosan.