Linear Sweep Voltammetric Determination Of Three Kinds Of Biomolecules

Biomolecules are very important components in all lives of the world, which play significant roles as information molecules in the research fields of life science, chemistry and medicine. Generally speaking, biomolecules are divided as protein, nucleic acids (NAs) and glycosaminoglycans (GAGs).In this paper, several bimolecules are selected for the investigation, which have no electrochemical signals themselves in the selected potential range. The electrochemical behaviors of organic molecule and its interaction with bimolecule are carefully studied by linear sweep voltammetry. Organic dye can be reduced on the mercury electrode and has a well-defined second order derivative linear sweep polarographic curve. After the addition of bimolecule, they can interact with each other and result in the changes of electrochemical response. The paper can be summarized as follows:1. The studies on the interaction of alizarin red S, arsenazo I and arsenazo III with protein.Alizarin red S, arsenazo I and arsenazo III are selected as electrochemical probes for the determination of protein. After the addition of protein into the solution, the peak current decreased apparently and the difference of the peak current was proportional to the concentration of protein. The conditions for the interaction were optimized. Under the optimal conditions, the working curve for protein was constructed. Synthetic samples were determined with satisfactory results and the stoichiometry of protein with organic dyes was calculated by the voltammetric data.2. The studies on the interaction of acridine orange, safranine T and victoria blue B with nucleic acids.The interaction of three organic dyes such as acridine orange, safranine T and victoria blue B with nucleic acids were studied. The conditions for the interaction were optimized. Under the optimal conditions, the working curve for nucleic acids was established. Synthetic samples were determined with satisfactory results and the stoichiometry of nucleic acids with organic dyes was calculated by the voltammetric data.3. The studies on the interaction of pyronine B and toluidine blue with chondroitin sulfate.The interaction of pyronine B and toluidine blue with chondroitin sulfate was studied. The conditions for the interaction were optimized. Under the optimal conditions, the decrease of reduction peak current was proportional to the concentration of chondroitin sulfate, which was further used for chondroitin sulfate and synthetic samples determination.