Molecular Diversity Of The Intestine Bacterial Community Of Anopheles Sinensis (Diptera: Culicidae)

Anopheles sinensis is the most important vector transmitted Malaria in China, they are numerous and widespread distributing.Diversity research of the intestinal bacterial community of An.sinensis,explore intestinal bacteria on the impact of the physiological function and ecological significance of An.sinensis,understand coevolution between An.sinensis and its symbiotic bacteria,provide new knowledge for species evolution ways.Finally,these bacteria could be a potential way of reseach for new mosquito control methods and controlling malaria.In this study,the bacterial communities in larvae gut,adults gut and different geographic populations of An.sinensist were analysed using Denaturing Gradient Gel Electrophoresis(DGGE)of amplified 16S rRNA sequences.Twenty eight different DGGE profiles of bacteria clones were obtained from the larvae gut.With sequence similarities and phylogenetic tree,five major phyla of bacteria were observed:Gammaproteobacteria,Flavobacteria,Actinobacteria, Betaproteobacteria and Firmicutes.Gammaproteobacteria and Actinobacteria represented 80%of the total clones.Gammaproteobacteria has 57.6%,mainly in the midgut bacterial community;Actinobacteria has 37.6%,they are the main bacterial community in the hindgut.Leucobacter and Chryseobacterium genus have 19%and 10.6%in the whole intestinal bacteria,they are preponderant bacterium in lavae gut.The results showed that the intestinal microbial flora of An.sinensist belongs to three major phyla of bacteria:Gammaproteobacteria,Flavobacteriaa,Actinobacteria. Gammaproteobacteria represented 59.8%of the total clones.Gammaproteobacteria are mainly bacterial community respectively in the midgut and hindgut,respectively has 54%and 65.6%.Enterobacter sp.(alignment similarity 99%) and Chryseobacterium meningosepticum represented 16.4%and 13.8%,predominant in adults gut.Research diversity of the intestinal bacterial community in different population of An.sinensis,the results showde:Enterobacter sp.and Chryseobacterium meningosepticum are all existting in different population,the presence of these bacteria with no geographical and ecological environment relationship.Through cluster analysis that intestinal bacterial of different geographic population of Anopheles sinensis can be divided into two types:the first type including laboratory populations,Wuhan in Hubei,Loudi in Hunan and Qionglai in Sichuan Loudi species, the other type including Quzhou in Zhejiang and Huangguang in Hubei species,the same type of the intestinal bacterial species have big similarity coefficients,there are some similarities.In addition to geographical distribution,climate external environment,eating different foodand other the various factors have an impact on the structure and content of the intestinal microbial flora.