Construction Of The Genomic Library Of Candida Albicans And The Screening Of The Yeast Genomic Library

Candida albicans is the major pathogen causing human fungal infections. It is asexual and diploid. C. albicans is not amenable to functional genomic strategies used for Saccharomyces cerevisiae, because of mating difficulties, its diploid nature and the lack of random insertional mutagenesis methods. Thus a genomic library of Candida albicans is necessary for identifying the genes'function and mechanisms of pathogeny of Candida albicans.In this project, we construct the genomic library of Candida albicans. Then we transform the genomic library of Candida albicans into Saccharomyces cerevisiae mutants to screen for genes that act similarly to dominant negative mutations.Based on the result of screening gene mutants having growth defects on salt medium of Saccharomyces cerevisiae, we transformed a genomic library of Saccharomyces cerevisiae that was constructed on a low-copy vector into the selected gene mutants. Thereby, we can screen the genes related to the mechanisms of salt-tolerance.