Induce Odontogenetic Differentiation Of Human Keratinocyte Stem Cells

Many ectodermal organs, such as teeth and feathers, all develop from epithelium and mesenchyme. The reciprocal interactions of epithelium-mesenchyme are regulators of morphogenesis and cell differentiation in the process of teeth growth. Therefore, we can deduce that the regeneration of tooth organs based on stem cells requires participation of stem cells derived from epithelium or mesenchyme. objective of this study is explore the potentail capacity of human keratinocyte stem cells for odontogenetic differetiaontion in order to find suitable human epithelium-original stem cells and regeneration conditions for tooth regeneration, and an appropriate kind of cell and regenerative model for tissue engineering of tooth regeneration. In our research, hKSCs were isolated,cultured and identified. We integrate hKSCs into mouse tooth germs mesenchymal with odontogenic potential at Embryonic day 13.5(E13.5) as recombinant, then transplant the recombinant to immuno-compromised mice sub-renal culture to find out if hKSCs can acquire the odontogenic competence and participate in the tooth development.Result of the recombinant culture shows that the recombinated tooth can develop into dental structures and possessed well-developed dentin; but hKSCs haven't induced into ameloblasts in morphologyo In order to fatherly establish and improve the technology of hKSCs as dental epithelial in the process of tooth regeneratin, we appended FGF8,Shh,BMP4 into the recombinated chimeric respectively. The results shows that the recombinated tooth contained FGF8 growth factor possesses complete tooth coronal structure (dental pulp,dentiiu enamel,ameloblasts).;the one appended with Shh is similar to chimeric without growth factor, and the one with BMP4 has distinct ossification .We conclude that hKSCs can support dental development as a role of epithelial; and appended FGF8 induce additionally facilitate they odontogenic differentiation.