Effect Of SFrp3 RNAi On Early Tooth Development In The Mouse

Both sFrp3 and Wnt5a showed strong expressions in the dental papilla mesenchyme.The addition of excess sFRPs to mesenchyme resulted in retardation of tooth bud formation and the subsequent development of smaller teeth.These suggest that sFrp3 as a Wnt antagonist is important in regulating tooth development.We use RNA interference to decrease the expression of sFrp3 in molar mesenchyme to study the effects of sFrp3on mouse early tooth development in order to understand the role of Wnt signaling in mouse early tooth development..The methods used include:produce lentivirus(LV-shsFrp3)by three-plasmid system,infect E11.5 molar teeth and E13.5 mesenchymal cells to detect tooth development.Our fidings indicate that after 2 days' in vivo culture,the molars reached the late bud stage,while the control's reached the early bud stage.After 4 days' in vivo culture,the molars reached the late cap stage,while the control's reached the early cap stage.After 7 days' in vivo culture,the molars formed dentin,while the control's didn't form dentin. After 8 days' in vivo culture,the odontoblasts and ameloblasts were more densed and mature than that of the control's.All in one word,the development of the molars infected with LV-shsFrp3 was about two or three days faster than the control's.The tooth development in vitro culture also illustrated this point.And the efficiency of tooth formation of the E13.5 recombinant(E13.5 mesenchyme+E10.5 epithelium)was higher(50%)than that of the control's(33%),but there was nothing different in tooth morphology and size.One reason for decreasing the expression of sFrp3 could accelerate the tooth development might be that it would activate both canonical Wnt pathway and the planar polarity pathways.We can conclude that Wnt signaling is important during the early tooth development.